Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 24)

Gene Pulser MXcell™ System Manual
15
3 Prepare Mammalian Cells
Preparation of cells is critical to the success of any electroporation experiment. This chapter
contains important information about how to run a successful experiment on the Gene
Pulser MXcell™ electroporation system. Refer to the following sections:
• Electroporation of Mammalian cells (page 15)
• Reagents and Solutions for Electroporation (page 17)
Preparation of Mammalian Cells
This section describes techniques for preparing electrocompetent mammalian cells, and
how to manipulate them after electroporation. Many of these techniques have been tested at
Bio-Rad Laboratories. The following list provides an overview of the major steps to run an
electroporation experiment using the Gene Pulser MXcell™ electroporation system system.
Refer to the pages listed to obtain more information.
• Harvest and count the cells (“Harvesting and Counting the Cells” on page 16).
• Prepare the cells for electroporation (“Preparing the Cells for Electroporation” on
page 16).
• Resuspend cells in Gene Pulser electroporation buffer and transfer them to the
electroporation plate (“Electroporation” on page 16)
• Insert electroporation plate into plate chamber (figure 5 on page 7)
• Create a new protocol, or select an existing protocol from the home screen and
press the
PULSE button to electroporate the cells (“Protocol Set-Up Option” on
page 20)
• Remove the electroporation plate and plate the cells in fresh cell culture plates. If
needed, combine the cells from different wells into the same culture plate. Assess
the transfection efficiency. (“Assessing Transfection Efficiency” on page 17)
For information about the factors that affect electroporation conditions, see page 41.