Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 34)

Pre-Set Protocols Option
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4. Edit a selected protocol.
Enter the changes and press the Save key to save the changes:
• To rename a protocol, press the CLEAR key to delete it, type in the new name.
press ENTER to confirm change.
• Change parameters of the protocol by selecting the desired parameters and
typing new parameters
• Press ENTER to confirm the selection
5. Delete a selected protocol. Press the DELETE key. At the screen prompt, press the
DELETE key again.
6. Press Save to save the changes in the protocol (page 28).
7. Press the PULSE button to electroporate the sample.
TIP: Once you start a protocol, you can stop the experiment if needed. To stop a
protocol, press and hold the
PULSE button. When the protocol stops, the screen
displays the last pulse (see “Last Pulse Option” on page 26).
Pre-Set Protocols Option
Choose an existing protocol to run from the Pre-Set Protocols list. Save any protocol as
a user protocol for future experiments. Run the protocol, or edit it and then run it.
Bio-Rad Laboratories scientists have developed twenty-one optimized pre-set protocols so
you can quickly run an experiment. These protocols optimize typical electroporation
conditions, starting with known parameters or starting with unknown parameters.
To run a pre-set protocol, follow these instructions:
1. Select Pre-Set Protocol in the home screen.
Press ENTER to confirm the selection.
2. Select for a pre-set protocol from the list.
Press and release the up and down keys to move from one protocol to the next. Press
and hold the arrow keys scroll from screen to screen. Press
ENTER to confirm the
selection.
NOTE: For detailed information about the parameters and plate setup for each
pre-set protocol, see “Program and Run the System” on page 19.
Pre-Set Protocols:
1.
Opt mini 96 well/ Sqr.Exp
2. Opt mini 96 well/ Sqr
3. - Opt mini 96 well/ Exp
4. - Opt 96 well/ Sqr, NP, D
5. - 96 well/ Exp
6. 24 well/ Exp
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