Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 26)

Reagents and Solutions for Electroporation
17
Assessing Transfection Efficiency
To assess the efficiency of a transfection experiment, several techniques can be used:
Fluorescently labeled siRNAs can be used to determine the transfection efficiency for siRNA
delivery. Transfection efficiency can be measured by fluorescence microscopy or by flow
cytometry. For plasmid delivery the transfection efficiency can be determined by
electroporating plasmids expressing reporter genes such as GFP or
β-galactosidase.
If you electroporate a mammalian cell line for the first time, choose growth conditions
following the electroporation that are based on other experiments with those cells or with
other similar cells. Reagents and Solutions for Electroporation
This list includes recommended reagents and solutions for running an electroporation
experiment:
• Gene Pulser electroporation buffer (catalog #165-2676 and 165-2677)
• Growth medium with FBS and necessary additives
• Trypsin-EDTA
• Sterile PBS: 137 mM NaCl, 2.7 mM KCl, 9.5 mM sodium phosphate, pH 7.3
Reagents and Solutions for Electroporation
This list includes recommended reagents and solutions for running an electroporation
experiment:
• Gene Pulser electroporation buffer (catalog #165-2676 and 165-2677)
• Growth medium with FBS and necessary additives
• Trypsin-EDTA
Sterile PBS: 137 mM NaCl, 2.7 mM KCl, 9.5 mM sodium phosphate, pH 7.3