Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 49)

Gene Pulser MXcell™ System Manual | Pre-Set Protocols
40
Uniform 12 well/ Exp,Sqr
These are parameters that vary in each well for a 12-well plate. Except for the larger well
size, the protocol is the same as “Uniform 96 well/ Exp, Sqr” on page 39.Mixed 96 well/
Exp,Sqr
Table 16 shows the parameters that vary in well sets for a 96-well plate. The protocol varies
in a wide range of starting condition for exponential and square waveforms in well sets with
alternating rows of exponential (250 V, 350 μF) and square waves (250 V, 20 ms).
Mixed 24 well/ Exp,Sqr
These are parameters that vary in each well for a 24-well plate. Except for the larger plate
size, the protocol is the same as “These are parameters that vary in each well for a 12-well
plate. Except for the larger well size, the protocol is the same as “Uniform 96 well/ Exp, Sqr”
on page 39.Mixed 96 well/ Exp,Sqr” on page 40.
Mixed 12 well/ Exp,Sqr
These are parameters that vary in each well for a 12-well plate. Except for the larger plate
size, the protocol is the same as “These are parameters that vary in each well for a 12-well
plate. Except for the larger well size, the protocol is the same as “Uniform 96 well/ Exp, Sqr”
on page 39.Mixed 96 well/ Exp,Sqr” on page 40.
Table 16. Mixed 96well/Exp, Sqr conditions that vary across the plate.
Exp Sqr Exp Sqr Exp Sqr Exp Sqr Exp Sqr Exp Sqr
1 2 3 4 5 6 7 8 9 10 11 12
A 250 V
350 μF 250
V
20ms 250 V
350 μF 250 V
20 ms 250 V
350 μF 250 V
20 ms 250 V
350 μF 250 V
20 ms 250 V
350 μF 250 V
20
ms 250 V
350 μF 250 V
20
ms
B
C
D
E
F
G
H