Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 30)

Protocol Set-Up Option
21
Press the arrow keys to select a parameter, then press the alphanumeric keys to enter
a new value. Press
ENTER to confirm the entry.
TIP: Press Clear to delete the value.
This example shows some values for each parameter in the pulse with a square
waveform (
SQR):
NOTE: The P in the lower right corner indicates that the required parameters are
complete, and the PULSE button is ready.
TIP: You can also edit a well set by selecting Edit well Set? using the arrow
keys to move the cursor. Press ENTER to begin editing. For more information
about well set programming, see Step 5 "Select a waveform (
EXP or SQR)." on
page 22.
6. (Optional) Press Save to save the changes in the protocol (page 28).
7. Press the PULSE button to electroporate the sample.
TIP: Once you start a protocol, you can stop the experiment if needed. To stop a
protocol, press and hold the
PULSE button. When the protocol stops, the screen
displays the last pulse (see “Last Pulse Option” on page 26).
W ELL S ET P ROGRAMMING
To program a plate with well sets that run different protocols, follow these instructions:
1. Select Protocol Set-up in the home screen.
Press ENTER to confirm the selection.
2. Select a plate size.
Press the arrow keys to select a plate size, then press ENTER to accept the selection.
3. Select WELL SET.
Select WELL SET, then press ENTER to accept the selection.
4. Select a well set in the plate.
Use the left and right arrow keys to select the well set, or to move through parameter.
Press
ENTER to confirm the parameter entry.
96-Well Whole Plate
Edit well Set? (press ENTER)
Waveform: EXP – SQR
V: ---
C: ----
R: ?
96-Well Whole Plate
Edit well Set? (press ENTER)
Waveform: EXP – SQR
V: 500
C: 500
R: ?
D: 5.500
#. 3 S: 10.0 P
Plate:
96
24 12
Program:
WHOLE PLATE
WELL SET