Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits User Manual
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3. Add 400 µl of reconstituted standard to the first 1.5 ml tube with
0 µl of standard diluent. This is identified as S1 in the diagram below
and in the product insert provided with assay.
4.
Continue making serial dilutions of the standard as shown. After
making each dilution, vortex gently and change the pipet tip after every
transfer.
NOTE: Running an additional two 0 pg/ml blanks is strongly
recommended. The blank wells are useful for troubleshooting and
determining LOD. Use 50 µl of the appropriate standard diluent as
the blank sample. The 0 pg/ml points should be formatted as
blanks, not as points in the curve, when using Bio-Plex Manager
software. Formatting these wells as blanks automatically subtracts
the background MFI values from the reading, and may result in
negative MFI values in some wells. If negative MFI values are
undesirable, format the 0 pg/ml wells as background controls.
5. Keep the standards on ice until ready for use. Standards should be
used immediately and should not be frozen for future use.
Standard Dilution Series