Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits User Manual
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6. Select Step 4 (Enter Standards Info) to enter standards information.
Skip to the next step if you are using a downloaded protocol from
the website; this information will already be entered.
a)
Deselect the box labeled “same concentration values for all
analytes”.
b)
Select the first analyte from the pull-down cell.
c)
Click on the Enter Automatically button and then select the most
concentrated value (typically S1).
d)
Enter the value for the highest concentration. The information is
included on the product insert provided with the assay.
e)
Enter the dilution factor (usually 4) and select Calculate. The
concentrations for each replicate point of the standard will be
populated for the selected analyte.
f)
Repeat steps 6b through 6e for each analyte in the assay.
Plate Formatting Example
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