Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits User Manual
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a)
Click the Add Panel button
in the Select Analytes toolbar.
b)
Enter a name for the new panel in the top field. Select the “Bio-
Plex Pro” assay type from the pull down menu (only for version
5.0 or higher). Then click Add to add analytes.
c)
Enter the bead region number of the first analyte in the Region
field, and the analyte name in the Name field.
NOTE: The bead region number must be correct for proper
detection of analytes. Using the product insert provided with the
assay, confirm that this number is correct before proceeding.
d)
Click Add Continue to add the analyte to the panel and continue
adding more analytes. When you have entered your last analyte,
click the Add button to add it to the list.
e)
When you are finished creating the panel, click OK to save your
changes and return to the Select Analytes window.
4. After selecting the panel, move analytes to the Selected view by
choosing from analytes in the Available view using the Add button or
Add All button. Note that the analytes will already be entered in the
Selected view when using a downloaded protocol.
5. Select Step 3 (Format Plate) and click on the Plate Formatting tab.
Select the number of replicates and the auto fill direction. Then click
the
icon and drag the cursor over all the wells that contain
standards. Then click on the
icon and drag the cursor over the
wells that contain blanks. Repeat with
and
to identify all the
wells that contain controls and samples.
NOTE: If the protocol was downloaded from the website, the
standards will already be added to the plate format. Make any
necessary changes to the plate layout to match your plate
requirements.