Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits User Manual
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7. Select Step 5 (Enter Controls Info) to enter controls information. This
is where the concentration of the user-specified controls is entered
into the protocol.
a)
If necessary, deselect the box for same concentration values for
all analytes.
b)
Select an analyte from the pull down cell.
c)
Enter the description, concentration, and dilution information for
each user-specified control.
d)
Repeat steps 7b and 7c for each additional analyte in the assay.
8. Select Step 6 (Enter Sample Info) and enter sample information.
Remember to enter information for the appropriate dilution factor.
9. Select Step 7 (Run Protocol).
Acquire Data
1. Shake the assay plate at 1,100 rpm for 30 sec immediately before
acquiring data.
2. Visually inspect the plate and ensure that the assay wells are filled
with buffer prior to placing the plate in the Bio-Plex microplate platform.
3. Slowly remove the sealing tape and any plate cover before placing
the plate in the reader.
4. Select Step 7 (Run Protocol) and refer to the details in the
appropriate section below for your xMAP system software package:
Bio-Plex Manager Software version 4.1, 4.1.1 and 5.0
a)
Specify data acquisition for 100 beads per region.
b)
In Advanced Settings, confirm that the Bead Map is set to
25 region.
c)
In Advanced Settings, set the sample size to 50 µl.