Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits - Manual (Page 25)

Reacquire Data
It is possible to aquire data from a well or plate a second time using the
Rerun/Recovery mode located below Start in Step 7 (Run Protocol).
1. Check the wells where data will be acquired a second time. Any
previous data will be overwritten.
2. Remove the buffer by vacuum filtration and add 100 µl of assay buffer
to each well. Cover the filter plate with a new sheet of sealing tape.
3. Repeat Acquire Data steps 1-6 to acquire data a second time. The
data acquired should be similar to the data acquired initially;
however, the data acquisition time will be extended since fewer beads
are present in each well.
Luminex ® xPONENT ® Software
Luminex xPONENT software does not include the 25-bead map. Hence,
the 100-bead map is used. Refer to the detailed instructions in the
xPONENT software manual.
1. Calibrate with xMAP MagPlex Classification Microspheres and xMAP
Reporter Calibration Microspheres (sold by Luminex).
2. Verify with xMAP MagPlex Control Microspheres and xMAP Control
Microspheres (sold by Luminex).
3. Create the angiogenesis panel protocol. Make sure to include the
correct bead region numbers and target names (refer to the product
insert).
3. The 100-region map should be selected.
4. Set the gate settings to 10,000 (low) to 22,500 (high).
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