Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits - Manual (Page 14)

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Section 9
Assay Instructions
The following instructions apply to Bio-Plex Pro™ angiogenesis assays. All
of the necessary components are provided premixed for ease of use.
Plan Experiment
1. Assign which wells of a 96-well plate will be used for each standard,
control, and sample (see the example below).
2. Determine the total number of wells that will be used in the assay.
Include a 25% excess (or add 2 wells for every 8 wells used) to
ensure that enough diluted coupled beads, detection antibodies,
and streptavidin-PE are prepared.
Example Plate