Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits - Manual (Page 12)

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Section 8
Control Preparation (Optional)
Two vials of lyophilized angiogenesis control are provided in each Bio-Plex
Pro™ Angiogenesis assay. The preparation of high, medium, and low controls
is optional to monitor plate-to-plate variations. This section provides
instructions on how to reconstitute the Iyophilized controls (refer to the product
insert provided with the assay for reconstituted control values). The
reconstituted control can then be further diluted to prepare any concentration of
user-specified quality controls.
Reconstitute Angiogenesis Controls
To ensure optimal assay performance, angiogenesis controls should be
prepared in a manner consistent as that used to prepare the angiogenesis
standards.
1. Gently tap the glass vial containing the lyophilized angiogenesis
control on a solid surface to ensure the pellet is at the bottom.
2. Reconstitute 1 vial of lyophilized control with 800 µl of the appropriate
diluent. Do not use assay buffer to dilute controls. This is identified
as stock in the product insert provided with the assay.
3. Gently vortex 1–3 sec and incubate on ice for 30 min. Be consistent
with the incubation time to ensure optimal assay performance.
4. The reconstituted angiogenesis control should be further diluted to
create the desired QC samples in the same diluents specified in the
table below. Concentration of the reconstituted angiogenesis control
can be found in the product insert provided with the assay.