Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits User Manual
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Section 6
Sample Preparation
This section provides instructions for preparing samples derived from serum,
plasma, and tissue culture supernatant. For sample preparations not
mentioned here, consult the publications listed in Bio-Rad bulletin 5297,
available for download at discover.bio-rad.com
Serum and Plasma Samples
Note that for plasma samples, both EDTA plasma and citrate plasma are
acceptable. Avoid using hemolyzed samples.
1.
Collect and process the serum or plasma samples and assay immediately
or freeze at –20ºC. Avoid repeated freeze-thaw cycles.
2.
Centrifuge the samples at 13,200 rpm for 10 min at 4ºC to clear the
samples of precipitate. Alternatively, carefully filter the samples with a
0.8/0.22 µm dual filter to prevent instrument clogging.
3.
Immediately dilute 1 volume of sample with 3 volumes of sample diluent.
Keep the samples on ice until ready for use.
Tissue Culture Supernatant Samples
1.
Collect and process the tissue culture supernatant samples and assay
immediately or freeze at –20ºC. Avoid repeated freeze thaw cycles.
2.
If required, dilute the culture supernatant with culture medium.
Serum-free culture medium should contain carrier protein (such as BSA)
at a concentration of at least 0.5%. Keep the samples on ice until ready
for use.
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