Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits User Manual
Page 8
Vortexer
VWR brand mini-vortexer
Scientific Instruments Vortex-Genie 2 mixer
VWR catalog #58816-121
VWR catalog #58815-234
Reagent reservoir
Corning, Inc. Costar 50 ml reagent
reservoir 4870
Bio-Rad catalog #224-4872
Other materials
Pipets and pipet tips, sterile distilled
water, aluminum foil, absorbent paper
towels, 1.5 ml microcentrifuge tubes,
15 ml culture tubes
Section 5
Recommended Materials
6
Item
Ordering Information
Bio-Plex
®
suspension array system
(or Luminex System)
Bio-Rad catalog #171-000205
Bio-Plex validation kit
Bio-Plex calibration kit
Bio-Rad catalog #171-203001
Bio-Rad catalog #171-203060
Microtiter plate shaker
IKA-Schuttler MTS-4 shaker for 4
microplates or Lab-Line Model 4625
Plate Shaker (or equivalent, capable of
300-1,100 rpm)
IKA catalog #3208000
VWR catalog #57019-600
Filter plate vacuum apparatus
Bio-Rad Aurum™ vacuum manifold
IMPORTANT: The use of filter plate
manifolds other than the one specified
may result in diminished assay
performance; see section 8 for
instructions specific to this assay
Bio-Rad catalog #732-6470
Item
Ordering Information
Bio-Plex human serum diluent kit
For tissue culture samples, dilute
samples and standards with appropriate
tissue culture media
Bio-Rad catalog #171-305000
(1x96)
Bio-Rad catalog #171-305001
(10x96)
Bio-Plex Pro human angiogenesis
standards
Additional standards sold separately
(optional)
Bio-Rad catalog #171-D40003
(2 vials, lyophilized)
Bio-Rad catalog #171-D40004
(50 vials, lyophilized)