Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits - Manual (Page 3)

Section 1
Introduction
Angiogenesis is a critical component of various normal and pathological
processes. A regulated angiogenic process includes the growth of new
blood vessels for tissue repair, fetal development and female reproductive
cycle. An uncontrolled angiogenic growth contributes to a major
pathological component of diseases such as cancer, cardiovascular
disease, and rheumatoid arthritis. Many proteins involved in the
uncontrolled angiogenic growth are candidate drug targets relevant for the
development of cancer therapies. Developing these therapies involves
measurement of various angiogenesis biomarkers.
Bio-Plex Pro™ angiogenesis assays are magnetic bead-based multiplex
assays that allow the measurement of angiogenesis biomarkers in diverse
matrices including serum, plasma, and cell/tissue culture supernatant. The
multiplexing feature makes it possible to quantitate the level of multiple
angiogenesis targets in a single well of a 96-well microplate in just 3 hours,
using as little as 12.5 µl of serum or plasma, or 50 µl of culture
supernatant.
As one of the most recent additions to the Bio-Plex ® suspension array
system, these assays incorporate magnetic beads into their design. The
magnetic beads allow the use of an assay protocol similar to non-magnetic
Bio-Plex assays, with the option of using magnetic separation of wash
steps instead of vacuum filtration (and allows automation of many of the
steps).
Bio-Plex Manager™ software is recommended for Bio-Plex Pro
angiogenesis assays. Instructions for Luminex xPONENT software are
also provided. For instructions using other xMAP system software
packages, contact Bio-Rad Technical support or your Bio-Rad field
application specialist.
For a current listing of Bio-Plex angiogenesis assays, visit us on the Web at
www.bio-rad.com/bio-plex/
1