Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits - Manual (Page 26)

Section 11
Troubleshooting Guides
This troubleshooting guide addresses problems that may be encountered with
Bio-Plex Pro™ angiogenesis assays. If you experience any of the problems listed
below, review the possible causes and solutions provided. This will assist you in
resolving problems directly related to how the assay steps should be performed.
Poor assay performance may also be due to the Bio-Plex
® suspension array
reader. To eliminate this possibility, we highly recommend use of the Bio-Plex
validation kit. This kit will validate all the key functions of the array reader and assist
the user in determining whether or not the array reader is functioning properly.
Possible Causes Possible Solutions
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High Inter-Assay CV
Standards were not
reconstituted consistently Incubate the reconstituted
standards for 30 min on ice. Always
be consistent with the incubation
time and temperature.
Reconstituted standards and
diluted samples were not stored
properly Reconstituted standards and diluted
samples should be prepared on ice
as instructed. Prior to plating, the
reconstituted standards and diluted
samples should be equilibrated to
room temperature.
High Intra-Assay CV
Bottom of filter plate not dry
Dry the bottom of the filter plate with
absorbent paper towel (preferably
lint-free) to prevent cross-
contamination.