Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits - Manual (Page 20)

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1. Select Calibrate and confirm that the default values for
CAL1 and CAL2 are the same as the values on the Bio-Plex
calibration bead labels. Use the Bio-Plex low RP1
target value.
2. Select OK and follow the instructions for CAL1 and CAL2
calibration.
Bio-Plex Manager Software version 4.0, 4.1 and 4.1.1
This software versions permit calibration at either low or high
RP1 target values. The selection of which target value to use is
assay dependent. For Bio-Plex Pro angiogenesis assays,
calibrate using the low RP1 target value on the Cal 2 bottle. Re-
calibration is necessary between assays requiring different RP1
target values. Daily calibration is recommended before acquiring
data.
1. Select Calibrate and confirm that the default values
for CAL1 and CAL2 are the same as the values on the Bio-
Plex calibration bead labels. Use the Bio-Plex low RP1
target value.
2. Select OK and follow the instructions for CAL1 and CAL2
calibration.
Prepare Protocol
Lot-specific assay protocols are available for download at www.bio-
rad.com/bio-plex/standards. If the desired protocol is not available for
download, create a new protocol.
1. Open a new protocol by selecting File, then New from the main
menu. Locate the steps at the left of the protocol menu.
2. Select Step 1 (Describe Protocol) and enter information about the
assay.
3. Select Step 2 (Select Analytes) and choose the appropriate
angiogenesis panel name, if available in the menu. If the panel name
is not available, create a new panel: