Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits - Manual (Page 27)

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Possible Causes Possible Solutions
Pipetting technique Pipet carefully and slowly when
adding standards, samples,
detection antibodies, and
streptavidin-PE, especially when
using a multichannel pipet. Use a
calibrated pipet. Change pipet tip
after every volume transfer.
Reagents and assay components
were not equilibrated to room
temperature prior to plating All reagents and assay components
should be equilibrated to room
temperature prior to plating.
Contamination with wash
buffer during wash steps During the wash steps, be careful
not to splash wash buffer from one
well to another. Be sure that the
wells are filtered completely and that
no residual volume remains. Also,
be sure that the microplate shaker
setting is not too high. Reduce the
microplate shaker speed to minimize
splashing.
Slow pipeting samples and
reagents across the plate Sample pipeting across the entire
plate should take less than 4 min.
Reagent pipeting across the entire
plate should take less than 1 min.