Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits User Manual
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d)
In Advanced Settings, confirm that the default DD gate
values are set to 5000 (low) and 32000 (high).
e)
Select Start, save the .rbx file, and begin data acquisition.
Bio-Plex Manager Software version 4.0
Note that this version does not include the 25 Bead Map. Hence,
the 100 bead map is used.
a)
Specify data acquisition for 100 beads per region.
b)
In Advanced Settings, set the sample size to 50 µl.
c)
In Advanced Settings, confirm that the default DD gate
values are set to 5000 (low) and 32000 (high).
d)
Select Start, save the .rbx file, and begin data acquisition.
5. If acquiring data from more than one plate, empty the waste bottle
and refill the sheath bottle after each plate (if HTF not present). Select
Wash Between Plates
and follow the instructions for fluidics
maintenance. Then repeat the Prepare Protocol and Acquire Data
steps.
NOTE: Use the Wash Between Plates command after every plate run
to reduce the possibility of clogging the instrument.
6. When data acquisition is complete, select Shut Down
and follow
the instructions.