Bio-Rad Bio-Plex Pro Human Angiogenesis Reagent and Diluent Kits - Manual (Page 19)

Section 10
Data Acquisition
Bio-Plex Manager™ software is recommended for Bio-Plex Pro™
angiogenesis assays. Refer to the details in the appropriate section below
for your xMAP system software package. For instructions using other
xMAP system software packages, contact Bio-Rad technical support or
your Bio-Rad field applications specialist.
NOTE: To minimize protocol setup, lot-specific Bio-Plex Pro
angiogenesis assay protocols for Bio-Plex Manager version 4.0 and higher
are available for download at www.biorad.com/bio-plex/standards.
Prepare System
1. Empty the waste bottle and fill the sheath fluid bottle before starting
(if HTF not present). This will prevent fluidic system backup and
potential data loss.
2. Turn on the reader, XY platform, and HTF (if included). Allow the
system to warm up for 30 min.
3. Select Start up and follow the instructions to prepare the reader
to acquire data. If the system is idle for 4 hr without acquiring data, the
lasers will automatically turn off. To reset the 4-hour countdown, select
Warm up
and wait for the optics to reach operational
temperature.
Calibrate With Low RP1 Target Value
Calibrate using Bio-Plex calibration beads and target values. Bio-Plex Pro
angiogenesis assays are run using the low RP1 target value on the Cal 2
bottle. Refer to the details in the appropriate section below for your xMAP
system software package.
Bio-Plex Manager Software version 5.0 and highe r
These software versions require calibration only with the low RP1
target value. Daily calibration is recommended before acquiring data.
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