Dako Pascal - Manual (Page 13)

Pascal Handbook 13
Section 4
Operating Instructions
Set Up
1. Familiarize yourself with Pascal and its parts prior to operating.
2. Adjust the pan by matching the pan handles with the body handles.
Note If the outside of the pan is wet, Pascal will make a cracking noise. Any water in the
body may cause malfunctions.
3. Fill the pan with 500 mL of deionized water for performing the standard Target Retrieval
procedure.
4. Ensure the heat shield is in the center of the pan.
5. Place 1 to 4 Tissue-Tek
® containers each filled with 250 mL of suitable Target Retrieval
Solution in the pan. Alternatively, use plastic Coplin jars. Fill each plastic Coplin jar with
50 mL of Target Retrieval Solution. Place slides intended for the Target Retrieval
procedure in the Tissue-Tek
®
containers or Coplin jars.
6. Place a dry Pascal Quality Strip on top of the containers.
7. Position the lid on the pan and secure in place by aligning the OPEN arrow etched on
the lid’s surface with one of the white dots on the pan handle. Grip the lid handle and
rotate clockwise so that the CLOSE arrow is aligned with the white dot on the pan handle.
8. Place the weight (petcock) on the vent nozzle located on the Pascal chamber lid.
9. Turn the red main power switch to the ON position (I).
Programming
Pascal Factory Settings
Push the Display Set button to recall the following factory setting parameters on a new
instrument. These settings can be changed for individual applications. Refer to Changing
Factory Setting within this section for notes on making changes for specific applications.
Pascal Factory Settings
Factory Settings Temperature Time
SP1 125 ºC 30 seconds
SP2 90 ºC 10 Seconds
SP Limit 10 ºC n/a