Dako Pascal User Manual

Page 19

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Pascal Handbook

19

manual for both the factory setting parameters and the instructions on changing
Pascal settings.

2.

Place 500 mL water inside the Pascal pan followed by a 250 mL bath of water
containing a slide rack with 24 slides.
• Place a Steam Monitor Strip on the bath container.

• Close the Pascal lid and press the “Start/Stop” button.

3.

When the SP1 temperature is reached and the alarm sounds verify the digital
display temperature is 126–128 ºC and chamber pressure is 21–23.4 pounds per
square inch (psi). If these parameters are within this range then the Pascal should
be considered to be within calibration limits for the High Temperature Calibration
check point. If the temperature and the pressure are not within the above range,
perform the following adjustment:

• Turn the power switch to the off position.
• Turn it back on while pressing the Up

and Down

arrows at the same

time.

• Confirm all 6 LEDs are illuminated; this indicates that Pascal is in calibration

offset mode.

• Press the “Display Set” button one time.
• Adjust the digital display temperature reading by using Up

or Down

arrows to match the pressure that is displayed on the Pascal pressure gauge to
the corresponding temperature recorded on the Pressure-Temperature
Calibration Chart below.

Note The arrow button may need to be pushed and held with consistent

pressure until the temperature changes. Work quickly as the
temperature and pressure are decreasing.

Pressure-Temperature Calibration Chart

Pressure

Temperature

Pressure

Temperature

13.0 psi

118.0 ºC

18.9 psi

124.0 ºC

13.5 psi

118.5 ºC

19.5 psi

124.5 ºC

14.0 psi

119.0 ºC

20.0 psi

125.0 ºC

14.4 psi

119.5 ºC

20.5 psi

125.5 ºC

15.0 psi

120.0 ºC

21.0 psi

126.0 ºC

15.4 psi

120.5 ºC

21.6 psi

126.5 ºC

15.9 psi

121.0 ºC

22.2 psi

127.0 ºC

16.3 psi

121.5 ºC

22.8 psi

127.5 ºC

16.8 psi

122.0 ºC

23.4 psi

128.0 ºC

17.3 psi

122.5 ºC

23.9 psi

128.5 ºC

17.8 psi

123.0 ºC

24.5 psi

129.0 ºC

18.3 psi

123.5 ºC

25.1 psi

129.5 ºC

One pound force per square inch ( 1 psi) equates to 6.895 kPa (kiloPascals), conversion factor provided by United
Kingdom National Physical Laboratory.


4.

Turn the Power off, and wait for Pascal to cool down to room temperature, i.e.
<27 °C. Cold water can be used to reduce the device’s temperature for this step.

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