Dako Pascal - Manual (Page 21)

Pascal Handbook 21
Inconsistent/weak Target Retrieval performance
Ensure the integrity of the gasket. Check to see if pressure is consistent between at least
three separate runs. If pressure is dropping relative to standard operation based on a
consistent program setting, replace the gasket.
Poor morphology
Reduce programmed time interval; i.e., if set at 5 minutes for a given temperature,
reduce time to 30 seconds to decrease time at high temperature which may affect
morphology within certain tissue types.
Use appropriate Target Retrieval Solutions. High pH buffers (i.e. pH 9.9) have a
tendency to be harsh on tissue specimens and can contribute to poor morphology.
Adherence of specimen to glass slide
Start retrieval program at a higher temperature; i.e., 80 ºC as opposed to lower
temperature such as ambient or 37 ºC. This has been shown to reduce tissue adherence
failure.
Use appropriate Target Retrieval Solutions. High pH buffers have a tendency to be harsh
on tissue specimens and can contribute to tissue adherence failure.
Ensure tissue sections are mounted on silanized slides or (+) charged slides.
Ensure that tissue sections were mounted from a waterbath not containing gelatin or other
adhesive product.
Ensure that sectioned slides have been baked for 1 hour at 60
°C before processing.
Specifications
Model: S2800 (110–120 volts)
Pan Capacity: 3.2 liters
Weight: 3.7 kg (8.18 lbs.)
CE Listed