Dako Pascal - Manual (Page 15)

Pascal Handbook 15
End Program
1. When the temperature reaches the SP2 programmed temperature for the programmed
SP2 time limit, an audible beep will sound and all indicator-lights will be flashing. Press the
Start/Stop button to end the program. Confirm the pressure reads 0 psi, before unlocking
the Pascal lid.
Note If pressure is not at 0 psi when the SP2 audible alarm is reached but is low relative
to the pressure reached during the run, i.e. approximately 1-5 psi, the user should gently
tilt the weight (petcock) located on the lid to one side. This will release residual pressure
and enable a safe environment in which to open Pascal post run.
2. Slightly push down the lid and rotate counter-clockwise to the OPEN position. Turn the
On/Off switch to the Off position.
3. Check the Pascal Quality Strip for proper color (dark gray to charcoal black) and record
for quality control.
4. Remove container with slides and slowly rinse slides in running reagent grade water.
Slides should remain in the container while rinsing.
Note In order to maintain consistency between runs, Pascal should be started at the
same initial temperature for each run. This can be done in a variety of ways. The unit can
be cooled down by adding cool water to the pan to bring the temperature down to 37 ºC,
for example. Alternatively, the instrument can be brought up to 80 ºC by setting SP1 to
80 ºC. When the alarm sounds, load the instrument, program the run parameters and start
the run. This ensures consistency between runs. If only one run is performed per day, this
may not be an issue because the starting point will be close to the same each day, i.e.
ambient temperature.
Changing Factory Settings
1. Press the Display Set button until the SP1 LED illuminates. Use the up and down
arrows to set the SP1 temperature to the desired temperature. SP1 can be utilized
exclusively when the SP2 time is programmed at 0 minutes.
Note To speed up the time and temperature setting, push the second arrow while holding
the original direction arrow down.
Note Time Bypass feature: if Set Point 1 (SP1) time is programmed to 99:00 minutes,
Pascal will continuously maintain temperature without a time countdown. When using the
Time Bypass feature, the temperature cannot be set at greater than 100 ºC as a safety
precaution. If the bypass feature is set at 99:00 minutes at SP1, the SP2 function is
disabled.
2. Press the Display Set button again and use the up and down arrows to set the SP1
time to the desired program time.