Dako Pascal - Manual (Page 14)

Pascal Handbook 14
Pascal Setting Limits
These are the factory settings. SP1 and SP2 settings can be changed by the user.
DakoCytomation recommends performing Target Retrieval in Pascal at 120–125 ºC for
30 seconds–5 minutes. Recommended times may vary according to the application. Most
DakoCytomation applications recommend performing Target Retrieval in a pressure
chamber set at 125 ºC for 30 seconds.
Note The Pascal Quality Strip optimal performance (charcoal black color) is at 125 ºC and
relative pressure. Lower temperature and pressure will produce a lighter colored strip; this
should be consistent at the same temperature and pressure between runs.
Start Program
1. Press the Display Set button until the SP1 LED indicator illuminates.
2. Push the Start/Stop button to start the program.
3.
Push the down arrow to display the actual temperature.
Note Both the Heat On light and Actual temperature light should be illuminated. The time
required to reach the temperature programmed depends on many variables such as the
voltage source and frequency, the number of bath/slides in the chamber, and the altitude
at which the instrument is operated.
Note Upon reaching the programmed temperature, the Heat On LED will turn off and the
timer will start counting down. The instrument will overshoot the programmed temperature
by approximately 2–3 ºC, and then will slowly come down to the programmed temperature.
When the digital timer reaches 0, the audible alarm will sound.
4. When the alarm sounds, press the Start/Stop button and the SP2 LED will illuminate.
5. For quality control, immediately record the temperature and pressure.
Note For quality control, immediately record the temperature and pressure. The pressure
will vary with contents within Pascal and the SP1 temperature setting. The temperature
may read +0–3 ºC above set SP1 temperature setting.
Minimum Maximum
SP Temperature 37 ºC 125 ºC
SP Time :01 second 98:59 minutes