5 liquid handling functions in detail, Liquid handling functions in detail – Metrohm 838 Advanced Sample Processor Manual User Manual

Page 69

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2.8 Dosing and liquid handling

Metrohm 838 Advanced Sample Processor, Operation

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2.8.5 Liquid handling functions in detail

Dosing

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Example:

DOS: 1.1 : 1.000 mL

Default port = 1

'Normal' dosing can be carried out by entering a volume. Automatic

cylinder filling is triggered neither before nor after the dosing process.

If the dosing piston reaches 'max. Volume' mark (10'000 impulses) dur-

ing the dosing process then the cylinder will be refilled.

Dosing a negative volume

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Example:

DOS: 1.2 : -1.000 mL

Default port = 1

If a negative value is set for a volume then the dosing process will take

part in the opposite direction, i.e. a liquid will be aspirated through the

given port. Automatic cylinder filling is triggered neither before nor after

the dosing process.

If the dosing piston reaches the zero mark during the dosing process

then the cylinder will be refilled.

Do not select any volume that is larger than the nominal cylinder vol-

ume. Aspiration should take place with a single piston stroke.

This function can be used for pipetting.

Filling

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Example:

DOS: 1.3 : fill mL

Default port = 2

Filling the cylinder can be carried out from a freely selectable port. After

the filling process the stopcock remains at the selected port.

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