YSI 600DW-B Sonde - Manual (Page 42)

Sondes Section 2
section 2.6.1 above for details. Failure to carry out the calibration properly can result in inaccurate readings,
particularly in water of very low turbidity.
Thoroughly rinse and dry the calibration cups for future use.
CHLOROPHYLL 1-POINT
Select Optic C -Chlorophyll from the Calibrate Menu, Chl µg/L and then 1-1 point.
NOTE: This procedure will zero your fluorescence sensor and use the default sensitivity for calculation of
chlorophyll concentration in µg/L, allowing quick and easy fluorescence measurements that are only semi-
quantitative with regard to chlorophyll. However, the readings will reflect changes in chlorophyll from site
to site, or over time at a single site.
To increase the accuracy of your chlorophyll measurements, follow the 2-point or 3-point calibration
protocols outlined in
Section 2.9, Sonde Menu.
Before making any field readings, carefully read Section 5.12, about chlorophyll that describes practical
aspects of fluorescence measurements.
To begin the calibration, place the correct amount of clear deionized or distilled water, from Tables 1, 2 and
3, into the YSI clear calibration cup provided. Immerse the sonde in the water
. Input the value 0 µg/L at
the prompt, and press Enter. The screen will display real-time readings that will allow you to determine
when the readings have stabilized. Activate the wiper 1-2 times by pressing 3-Clean Optics as shown on
the screen to remove any bubbles from the sensor. After stabilization is complete, press Enter to
“confirm” the calibration and then, as instructed, press Enter to return to the Calibrate menu.
Thoroughly rinse and dry the calibration cups for future use. For additional information related to
calibrating the chlorophyll sensor, see Sections 5.12.
RHODAMINE WT 2-POINT
Select Rhodamine from the Calibrate Menu and then 2-2-Point.
NOTE: One standard must be 0 ug/L in rhodamine WT, and this standard must be calibrated first.
To begin the calibration, place the correct amount (see Tables 1-4) of 0 standard (clear
deionized or
distilled water) into the calibration cup provided with your sonde and immerse the sonde in the water.
Input the value 0 ug/L at the prompt, and press Enter. The screen will display real-time readings that
will allow you to determine when the readings have stabilized. Activate the wiper 1-2 times by
pressing 3-Clean Optics as shown on the screen, to remove any bubbles. After stabilization is
complete, press Enter to “confirm” the first calibration and then, as instructed, press Enter to
continue.
Dry the sonde carefully and then place the sonde in the second rhodamine WT standard (100 ug/L is
recommended) using the same container as for the 0 ug/L standard. Input the correct rhodamine WT
concentration in ug/L, press Enter, and view the stabilization of the values on the screen in real-time.
As above, activate the wiper with the “3” key or manually rotate the sonde to remove bubbles. After
the readings have stabilized, press Enter to confirm the calibration and then press Enter to return to
the Calibrate menu.
YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-35