Operation, 1 setting up the instrument, Setting up the instrument – Leica Biosystems ST4040 User Manual
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Instruction Manual V 1.4 – 06/2009
6.2
Preparing the instrument for staining: inserting and filling the stations
•
Select a staining protocol.
(For examples, see Appendix 1: ‘Staining protocols’)
•
According to the selected staining protocol, determine the order of re-
agent and tap water stations.
•
Open all three lids.
The Leica ST4040 is equipped with a three-piece lid, thus e
n
-
abling the operator to open just one of the two small lids on the right
or left of the instrument for loading / unloading slide racks. This sys-
tem keeps the exposure of laboratory personnel to hazardous fumes
to a minimum.
•
Remove the cover screws from those positions where you have decid-
ed to install tap water stations (tap water stations can be installed from
positions 3 through 20).
•
Insert the tap water stations (yellow - see left) and the reagent stations
(white).
•
Make sure that all stations are inserted correctly; the stations must not
be jammed (overlapping rims!).
•
Add reagents according to selected staining protocol. - Do not exceed
the maximum volume or remain under the minimum volume!
•
Close the lid entirely.
6.
Operation
6.1
Setting up the instrument
To set up the instrument follow all instructions of chapter 6 step by
step.