Leica Biosystems ST4040 - Manual (Page 31)

31 Leica ST4040 – Linear Stainer
6. Operation
6.3 Staining in two rows
6.3.1 Doubling the specimen throughput
• For this type of application, the sequences of reagent / tap water sta-
tions in rows 1 and 2 must be identical.
• According to the station sequence selected for row 1 (see Chapter 6.2)
insert an identical sequence in row 2.
• Both rows are run with the same program.
6.3.2 Carrying out two different, but matching, staining protocols simultaneously
• Precondition: the program parameters of both protocols must be the
same (i.e. both rows are run with the same program).
• The two staining protocols are coordinated through the number of re-
agent stations and/or the concentration or dilution of the reagents (for
details, see Appendix 1
- ‘Staining protocols’).
Important note to chapters 6.3.1 and 6.3.2
• When staining in two rows, standard slide rack carriers, as for single
row staining, are used.
• Slide rack carriers located side by side in rows 1 and 2 are inserted
laterally reversed into the same notch of the conveyor frame (see left).
6.3.3 Carrying out two different staining protocols (two subsequent single loads)
• The two rows are never carried out simultaneously, i.e.: row 1 is run
with program 1 - row 2 with program 2, or vice versa.
In 6.3.3 the advantage of staining in two rows is that 2 different staining protocols, though not
run simultaneously, can be run one directly after another without any delay; whereas in a sin-
gle load model, stations would have to be rearranged, refilled etc. prior to being able to run the
second protocol.