LaMotte SMART Spectro Spectrophotometer - Manual (Page 165)

PREPARATION OF *TMK INDICATOR
þNOTE: Prepare *TMK Indicator daily. Keep out of direct sunlight.
1. Fill test tube (0778) to the 10 mL line with *Propyl Alcohol (4863).
2. Add one *TMK Tablet (4862).
3. Use tablet crusher (0175) to completely crush tablet.
4. Cap and mix. Shake vigorously for 30 seconds.
PROCEDURE
Use universal sample holder.
1. Press and hold
ON button until spectrophotometer turns on.
2. Scroll and select PROGRAMMED TESTS.
3. Scroll and select ALL TESTS (or another sequence containing
57 Mercury
) from TESTING MENU.
4. Scroll to and select 57 Mercury from menu.
5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample.
6. Insert the tube into chamber, close lid and select SCAN BLANK.
7. Remove the tube from spectrophotometer.
8. Use the 1.0 mL pipet (0354) to add 3 mL of *Acetate Buffer (4864). Cap
and mix.
9. Use the 0.5 mL pipet (0353) to add 0.5 mL of prepared *TMK Indicator.
Cap and mix.
10. Wait one minute.
11. Insert tube into chamber, close lid and select SCAN SAMPLE. Record
result as ppm Mercury.
12. Press
OFF button to turn the spectrphotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
þNOTE: For best possible results, a reagent blank should be determined to
account for any contribution to the test result by the reagent system. To
determine the reagent blank, follow the above test procedure using distilled
or deionized water. This test result is the reagent blank. Subtract the reagent
blank results from all subsequent test results of unknown samples. It is
recommended that a reagent blank be determined each time *TMK
Indicator is prepared.
Mercury 1/2 SMART SPECTRO TEST PROCEDURES 05.04