LaMotte SMART Spectro Spectrophotometer - Manual (Page 186)

CARBOHYDRAZIDE PROCEDURE
Use universal sample holder
1. Press and hold
ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS.
3. Scroll to and select ALL TESTS (or another sequence containing
14 c-hydrazide
) from TESTING MENU.
4. Scroll to and select 14 c-hydrazide from menu.
5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample.
6. Insert the tube into chamber, close lid and select SCAN BLANK.
7. Remove the tube from spectrophotometer.
8. Add 3 drops of *DEHA Reagent #1 (4791). Swirl to mix.
9. Add 3 drops of *DEHA Reagent #2 (4792). Swirl to mix.
10. Add 3 drops of *DEHA Reagent #3 (4793). Invert 3 times to mix.
11. Insert the tube into chamber. Close lid.
12. Wait 15 minutes. Do not open the lid during the waiting time. The
reaction is photosensitive.
13. Remove tube from chamber. Invert 2 times to mix.
14. Immediately insert tube into chamber, close lid and select SCAN SAMPLE.
Read within 30 seconds. Record result in ppm carbohydrazide.
15. Press
OFF button to turn the spectrophotometer off or press EXIT button
to exit to a previous menu or make another menu selection.
þNOTE: For best possible results, a reagent blank should be determined to
account for any contribution to the test result by the reagent system. To
determine the reagent blank, follow the above test procedure to scan a
distilled or deionized water blank. Then follow the above procedure to
perform the test on a distilled or deionized water sample. This test result is
the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only
when a new lot number of reagents is obtained.
SMART SPECTRO TEST PROCEDURES 05.04 Oxygen Scavengers 3/6