LaMotte SMART Spectro Spectrophotometer - Manual (Page 62)

AMMONIA-NITROGEN–HIGH RANGE
NESSLERIZATION METHOD • CODE 3642-SC
QUANTITY CONTENTS CODE
30 mL Ammonia Nitrogen Reagent #1 V-4797-G
2 x 30 mL * Ammonia Nitrogen Reagent #2 *V-4798-G
1 Pipet, 1 mL, plastic 0354
*WARNING: Reagents marked with a * are considered to be potential health hazards. To view
or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Ammonia nitrogen is present in various concentrations in many surface and
ground water supplies. Any sudden change in the concentration of ammonia
nitrogen in a water supply is cause for suspicion. A product of microbiological
activity, ammonia nitrogen is sometimes accepted as chemical evidence of
pollution when encountered in natural waters.
Ammonia is rapidly oxidized in natural water systems by special bacterial
groups that produce nitrite and nitrate. This oxidation requires that dissolved
oxygen be available in the water. Ammonia is an additional source of nitrogen
as a nutrient which may contribute to the expanded growth of undesirable
algae and other forms of plant growth that overload the natural system and
cause pollution.
APPLICATION: Drinking, surface, and saline waters; domestic and industrial
wastes.
RANGE: 0.00–4.00 ppm Ammonia Nitrogen
METHOD: Ammonia forms a colored complex with Nessler’s Reagent in
proportion to the amount of ammonia present in the sample.
Rochelle salt is added to prevent precipitation of calcium or
magnesium in undistilled samples.
SAMPLE
HANDLING &
PRESERVATION: Ammonia solutions tend to be unstable and should be
analyzed immediately. Sample may be stored for 24 hours at
4°C or 28 days at –20°C.
INTERFERENCES: Sample turbidity and color may interfere. Turbidity may be
removed by a filtration procedure. Color interference may be
eliminated by blanking the instrument with a sample blank.
SMART SPECTRO TEST PROCEDURES 05.04 Ammonia-Nitrogen–HR 1/2