Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 21)

Time Constant Protocol Detail screen
Fig. 3.4. Time Constant Protocol Detail Screen. This screen shows the parameters that may be
specified for exponential decay electroporation when the time constant is designated. Entering
the cuvette size is optional and is for user information only.
• Use the Up and Down Arrow keys to highlight the parameter value space for voltage or time
constant. Use the alpha-numeric keypad to input the desired value, or use the Right and Left Arrow
keys to increase or decrease the parameter value by the smallest allowable increment. Use the
Delete or Clear keys to correct entries. When the desired value has been specified, press Enter. If a
value outside the limits of the Gene Pulser Xcell is selected, the value in the field will default to the
closest permitted value. Entering a value for cuvette size is optional and is for user information only.
• When the necessary parameter values have been specified, a flashing “P” appears in the character
space in the lower right corner of the LCD display indicating that the pulse button on the Gene
Pulser Xcell is active and that a pulse may be delivered.
• Press the Pulse button to deliver a pulse. When the Pulse button is depressed, the LCD display will
blank then show “Pulsing”. Upon completion, a tone will sound and the pulse measurements will be
displayed on the Protocol Results screen (see Figure 3.7, Section 3.3.5).
• Use the Left and Right Arrow keys to toggle between the Protocol Results screen and the last
Protocol Detail screen.
• With the Protocol Detail screen on the LCD display another pulse can be delivered using the same
pulse parameters. To change the pulse conditions, press Enter; the cursor appears in the voltage
parameter value. The parameters may be changed as described above.
• To save the pulse parameters, see Section 3.3.6.
• To review previously delivered pulses, see Section 3.8.
3.3.4 Electroporation using Square Wave Pulses
See Section 4.2 for a discussion of electroporation using square wave pulses. Use Gene Pulser Xcell to
deliver square wave pulses as follows.
• From the Home screen (Figure 3.2), press 3 or use the Up or Down Arrow keys to highlight
“Square wave protocol”, then press Enter. The Square Wave Protocol Detail screen appears
(Figure 3.5).
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P R O T O C O L D E T A I L : T I M E C O N S T A N T
V o l t a g e ( V ) X X X X
T i m e c o n s t a n t ( m s e c ) X X X X . X
C u v e t t e ( m m ) X