Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 40)

3.6 Last Pulse
From the Home screen (Figure 3.2), press 6 or use the Up or Down Arrow keys to select “Last pulse”,
then press Enter to display the Protocol Detail screen showing the last pulse delivered. This is also
accessible after the instrument has been turned off and restarted.
3.7 Optimize Operation
The Optimize mode allows the user to program a voltage that incrementally increases or decreases
when the Right and Left Arrow keys are pressed. This mode operates similarly to Manual Protocols
(Section 3.3.2, 3.3.3, and 3.3.4) but requires that a voltage increment be specified.
• Access this screen from the Home screen (Figure 3.2) by pressing 7 or use the Up or Down Arrow
keys to highlight “Optimize” then press Enter. The Select Method screen appears (Figure 3.17).
• Press 1–3 or use the Up and Down Arrow keys to highlight the number corresponding to optimizing
voltage by delivering (i) an exponential decay pulse (“Exponential protocol”), (ii) an exponential decay
pulse specifying the time constant (“Time constant protocol”), or (iii) a square wave pulse (“Square
wave protocol”). Press Enter to make a selection and to view the appropriate Optimize screen.
Select Method screen
Fig. 3.17. Select Method screen.
• When “Exponential protocol” is selected, the Exponential Decay Protocol Optimize screen appears
(Figure 3.25).
• When “Time constant protocol” is selected, the Time Constant Protocol Optimize screen appears
(Figure 3.26).
• When “Square wave protocol” is selected, the Square Wave Protocol Optimize screen appears
(Figure 3.27).
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S E L E C T M E T H O D
1 . E x p o n e n t i a l p r o t o c o l
2 . T i m e c o n s t a n t p r o t o c o l
3 . S q u a r e w a v e p r o t o c o l