Bio-Rad Gene Pulser Xcell™ Electroporation Systems User Manual
Page 32
3.5 User Protocols
User Protocols enable users to store their own protocols for the Gene Pulser Xcell. Up to 144
electroporation protocols may be programmed and stored. (12 users (User Names in the User
Directory) each with 12 protocols per user (User Protocols).)
A new User Protocol may be created either by:
•
Creating a new protocol by entering all new parameters in manual mode (Sections 3.3.2, 3.3.3,
and 3.3.4).
•
Modifying (editing) a Pre-set protocol (Section 3.4.3).
•
Creating a new protocol as a User Protocol (Section 3.5.3).
•
Modifying (editing) an existing User Protocol (Section 3.5.4).
Protocols created or modified in the User Protocols menu may be used without being saved (Section 3.5.4).
3.5.1 Using a User Protocol (Quick Guide)
•
From the Home screen, press 5, then Enter to open the User Protocols menu and to show the first
User Directory screen. Use the Right and Left Arrow keys to toggle between the two screens.
•
Press 1–12 to highlight the desired name; press Enter to select that name and to show the first
User Protocols screen. Use the Right and Left Arrow keys to toggle between the two screens.
•
Press 1–12 to highlight the desired protocol name; press Enter to select that protocol and to show
the Protocol Detail screen. The Pulse button on the Gene Pulser Xcell is now active.
•
Press the Pulse button to electroporate the sample.
•
Press the Back key to return to the Protocol Detail screen and to deliver another pulse.
3.5.2 Creating a New User Name
A new User Name can be created when the space adjacent to that number on the User Directory
screen is clear (Figure 3.15). If it is necessary to delete a User Name or a User Protocol, see Section
3.5.5.
Create a new User Name in the User Name screen as follows.
•
From the Home screen (Figure 3.2), press 5 or use the Up and Down Arrow keys to highlight “User
Protocols”, then press Enter to view the User Directory screen (Figure 3.15).
•
Use the Right and Left Arrow keys to toggle between the two User Directory screens. Press 1–12
or use the Up or Down Arrow keys to highlight a position without an entry. Press Enter to select
the position.
•
Use the alpha-numeric keypad to type in the desired User Name. A name can have up to ten
characters. Press Save when complete. The new User Name is saved.
3.5.3 Creating a New User Protocol
This section describes how to create a new User Protocol from the User Protocols screen and save the
protocol. A new User Protocol can be created when the space adjacent to that number on the User
Protocol screen is clear (Figure 3.16). Section 3.5.4 describes how to edit (change) an existing User
Protocol and save the changes.
26