Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 30)

Pre-set Protocol: CHO
Fig. 3.14. Protocol Detail Screen for CHO cells in the Pre-set Protocols menu.
Table 3.4. Optimized settings found in the Pre-set protocols function of the Gene
Pulser Xcell.
Pre-set
Protocol
PL C PC Cuvette Cell vol
Designation Cells Pulse type (msec) (µF) (ohm) V (cm) (µl)
Mammalian 1 CHO Square wave 15 160 0.2 100
Mammalian 2 COS7 Square wave 20 110 0.2 100
Mammalian 3 3T3 Exponential decay 500
∞
160 0.2 100
Mammalian 4 293 Square wave 25 110 0.2 100
Mammalian 5 HeLa Exponential decay 500
∞
160 0.2 100
Mammalian 6 BHK21 Square wave 25 140 0.2 100
Mammalian 7 A549 Square wave 10 150 0.2 100
Mammalian 8 CV1 Square wave 25 100 0.2 100
Mammalian 9 K562 Exponential decay 1000
∞
155 0.2 100
Mammalian 10 HL60 Square wave 25 140 0.2 100
Mammalian 11 Jurkat Exponential decay 1000
∞
140 0.2 100
Mammalian 12 HuT78 Square wave 25 130 0.2 100
Bacterial 1 E coli Exponential decay 25 200 1800 0.1 20
Bacterial 2 25 200 2500 0.2 20–40
Bacterial 3 25 200 3000 0.2 20–40
Bacterial 4 A tumefaciens Exponential decay 25 200 2400 0.1 20
Bacterial 5 P aeruginosa Exponential decay 25 200 2500 0.2 100
Bacterial 6 S aureus Exponential decay 25 100 2900 0.2 50
Bacterial 7 B cereus Exponential decay 50 200 1000 0.2 100
Bacterial 8 S pyogenes Exponential decay 25 200 2100 0.2 200
Bacterial 9 L plantarum Exponential decay 25 400 2000 0.2 40
Fungal 1 S cerevisiae Exponential decay 25 200 1500 0.2 40
Fungal 2 25 200 2500 0.4 80
Fungal 3 S pombe Exponential decay 25 200 2300 0.2 40
Fungal 4 C albicans Exponential decay 25 200 1500 0.2 40
Fungal 5 P pastoris Exponential decay 25 200 2000 0.2 40
Fungal 6 D discoideum Square wave 1.0 10 1000 0.4 800 2 pulses
24
P R O T O C O L D E T A I L : C H O
V o l t a g e ( V ) 1 6 0
P u l s e l e n g t h ( m s e c ) 1 5 . 0 0
N u m b e r o f p u l s e s 1
P u l s e i n t e r v a l ( s e c ) 0 0 . 0
C u v e t t e ( m m ) 2
P