Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 36)

• When “Square wave protocol” is selected, the Square Wave Protocol Detail screen appears
(Figure 3.20) with the User Name on the first line.
Square Wave Protocol Detail Screen
Fig. 3.20. Square Wave Protocol Detail screen
• Use the Up and Down Arrow keys to scroll through the parameter value spaces on the
appropriate Protocol Detail screen. Use the alpha-numeric keypad to input the desired value, or
use the Right and Left Arrow keys to incrementally increase or decrease the parameter value. Use
the Delete or Clear keys to correct entries. When the desired value has been specified, press Enter.
If a value outside the limits of the Gene Pulser Xcell is selected, the value in the field will default to
the closest permitted value (see Tables 3.1, 3.2, and 3.3). Entering a value for cuvette size is
optional and is for user information only.
• When the necessary parameter values have been specified, a flashing “P” appears in the character
space in the lower right corner of the LCD display indicating that the pulse button on the Gene
Pulser Xcell is active and that a pulse may be delivered.
• Press the Pulse button to deliver a pulse. When the Pulse button is depressed, the LCD display will
blank then show “Pulsing”. Upon completion, a tone will sound and the pulse measurements will
be displayed on the Protocol Results screen (see Section 3.3.5).
• To save the pulse parameters, with the Protocol Detail screen on the LCD, press Save.
• The User Protocols screen will appear with the cursor flashing on the first character space of the
selected position. Type in the name using the alpha-numeric keypad (alphabetic entries are the
default; press Shift to toggle between alphabetic and numeric entries). Press Save or Enter when
complete. The protocol is saved in the location named in the User Protocols screen.
3.5.4 Modifying a User Protocol
A User Protocol may be modified (changed) as follows.
• To enter the User Protocols screen from the Home screen (Figure 3.2):
• Press 5 or use the Up and Down Arrow keys to highlight “User Protocols”, then press Enter
to view the first User Directory screen (Figure 3.15).
• Use the Right and Left Arrow keys to toggle between the two User Directory screens. Press
1–12 or use the Up and Down Arrow keys to highlight the desired User Name. Press Enter to
select the name and to view the first User Protocols screen (Figure 3.16).
• Press 1–12 or use the Up, Down, Left, and Right Arrow keys to highlight the desired User Protocol
to be edited. Press Enter to view the Protocol Detail screen.
L a b g u y : P r o t o c o l :
V o l t a g e ( V ) X X X X
P u l s e l e n g t h ( m s e c ) X X X . X X
N u m b e r o f p u l s e s 1
P u l s e i n t e r v a l ( s e c ) 0 0 . 0
C u v e t t e ( m m ) X
30