And of (b) buffers of napo – Bio-Rad Gene Pulser Xcell™ Electroporation Systems User Manual

Page 57

Advertising
background image

Fig. 5.1. Resistance of solutions (A) NaCl and MgCl

2

and of (B) buffers of NaPO

4

at pH 6.1 and 7.3

and HEPES at pH 7.5. Resistance was measured in 0.2 cm cuvettes containing either 40 µl or 200
µl of solution at room temperature.

51

Concentration (mM)

Concentration (mM)

0

2

4

6

8

10

0

2

4

6

8

10

4000

3500

3000

2500

2000

1500

1000

500

0

3500

3000

2500

2000

1500

1000

500

0

B

A

Sample resistance (ohms)

Sample resistance (ohms)

NaCI (40 µl)

NaCI (200 µl)

MgCI

2

(40 µl)

MgCI

2

(200 µl)

PO

4

6.1, 40 µl

PO

4

6.1, 200 µl

PO

4

7.3, 40 µl

PO

4

7.3, 200 µl

HEPES, 7.5, 40 µl

HEPES, 7.5, 200 µl

Advertising