Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 28)

3.4.2 Electroporation using Pre-set Protocols
There are nine Pre-set Bacterial Protocols, six Pre-set Fungal Protocols, and 12 Pre-set Mammalian
Protocols. These protocols are pre-programmed with the optimal parameters for the given organism.
Use the Pre-set Protocols as follows.
• From the Home screen, press 4 or use the Up and Down Arrow keys to highlight “Pre-set
Protocols”, then press Enter to select and to show the Pre-set Protocols screen (Figure 3.12).
• Press 1–3, or use the Up and Down Arrow keys, to highlight Bacterial, Fungal, or Mammalian
Pre-set Protocols, then press Enter to select.
• Use the alpha-numeric keypad or the Up and Down Arrow keys to scroll through the list of names.
For the Bacterial and Mammalian Pre-set Protocols, use the Right and Left Arrow keys to toggle
between the two screens. When the number corresponding to the desired name is highlighted,
press Enter to select and to view the Protocol Detail Screen showing the electroporation parameters
for that protocol. A flashing “P” in the character space in the lower right corner of the LCD display
indicates that the Pulse button is active.
• For example, from the Pre-set Protocols screen, press 3 to highlight “Mammalian”, then press
Enter to select and to bring up the first Pre-set Mammalian Protocols screen with the names of
six pre-set mammalian protocols (Figure 3.13). Press the Right and Left Arrow keys to toggle
between the two Mammalian Pre-set Protocols screens. Use the alpha-numeric keypad or the
Up and Down Arrow keys to scroll through the list of names. When the desired name on the
Mammalian Pre-set Protocols screen is highlighted, press Enter to select that protocol and to
view the Protocol Detail Screen showing the electroporation parameters for that protocol. For
example, from the Mammalian Pre-set Protocols screen, press 1, then Enter to bring up the
Protocol Detail Screen for CHO cells in a 2 mm cuvette (Figure 3.14).
• Press the Pulse button to deliver a pulse. When the Pulse button is depressed, the LCD display will
blank then show “Pulsing”. Upon completion, a tone will sound and the pulse measurements will be
displayed on the Protocol Results screen (see Section 3.3.5).
• Use the Left and Right Arrow keys to toggle between the Protocol Results screen and the last
Protocol Detail screen.
• With the Protocol Detail screen on the LCD display another pulse can be delivered using
the same pulse parameters. To change the pulse conditions, press Enter; the cursor
appears in the voltage parameter value. The parameters may be changed as described in
Section 3.4.3.
• To review previously delivered pulses, see Section 3.8.
22