Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 22)

• The following combination of parameters may be entered:
Pulse length + Voltage
Pulse length + Voltage + Number of pulses + Pulse interval
Square Wave Protocol Detail screen
Fig. 3.5. Square Wave Protocol Detail Screen. This screen shows the parameters that may be speci-
fied for square wave electroporation. Entering the cuvette size is optional and is for user informa-
tion only.
Electroporation using square waves may be used to deliver a series of multiple pulses to the cells. This
is specified by the parameter “Number of pulses.” The time between each pulse is specified as the
“Pulse interval.” The default values for the number of pulses and pulse interval are 1 and 0, respectively;
it is only necessary to enter other values if more than one pulse is to be delivered. See Section 4.2 for a
discussion of pulse parameters.
The pulse parameters may be selected in any order. However, the set voltage will determine whether
the high voltage or the low voltage circuit is to be used. Consequently, the set voltage will limit the
ranges of the pulse length and pulse interval as indicated in Table 3.3. If values for the second and third
parameters are chosen that are outside the range of the system, the values will default to the closest
allowable values.
Table 3.3. Pulse length and pulse interval ranges for the high voltage / low
capacitance circuit and the low voltage / high capacitance circuit of the Gene
Pulser Xcell for delivering a square wave pulse.
Voltage (V) Pulse Length Pulse Interval No. Pulses (max)
LV Circuit* 10–500 0.05–100 ms 0.1–10 s 10
HV Circuit 200–3000 0.05–5 msec 5–30 s 2
*Use of the low voltage circuit requires that the CE Module be in place.
• Use the Up and Down Arrow keys to scroll through and highlight the parameter value space for
voltage, pulse length, number of pulses, or pulse interval. Use the alpha-numeric keypad to input
the desired value, or use the Right and Left Arrow keys to increase or decrease the parameter
value by the smallest allowable increment. Use the Delete or Clear keys to correct entries. When
the desired value has been specified, press Enter. If a value outside the limits of the Gene Pulser
Xcell is selected, the value in the field will default to the closest permitted value. Entering a value for
cuvette size is optional and is for user information only.
P R O T O C O L D E T A I L : S Q U A R E W A V E
V o l t a g e ( V ) X X X X
P u l s e l e n g t h ( m s e c ) X X X . X X
N u m b e r o f p u l s e s 1
P u l s e i n t e r v a l ( s e c ) 0 0 . 0
C u v e t t e ( m m ) X
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