Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 23)

• When the necessary parameter values have been specified, a flashing “P” appears in the character
space in the lower right corner of the LCD display indicating that the pulse button on the Gene
Pulser Xcell is active and that a pulse may be delivered.
• Press the Pulse button to deliver a pulse. When the Pulse button is depressed, the LCD display will
blank then show “Pulsing”. Upon completion, a tone will sound and the pulse measurements will be
displayed on the Protocol Results screen (see Figure 3.8, Section 3.3.5).
• Use the Left and Right Arrow keys to toggle between the Protocol Results screen and the last
Protocol Detail screen.
• With the Protocol Detail screen on the LCD display another pulse can be delivered using the same
pulse parameters. To change the pulse conditions, press Enter; the cursor appears in the voltage
parameter value. The parameters may be changed as described above.
• To save the pulse parameters, see Section 3.3.6.
• To review previously delivered pulses, see Section 3.8.
3.3.5 Results Screens
After delivering a pulse, the LCD displays the results on a Protocol Results screen. This screen shows
the results in both graphic and tabular form. Figures 3.6, 3.7, and 3.8 show examples of the results
from an exponential decay pulse, an exponential decay pulse in which the time constant was specified,
and a square wave pulse, respectively.
Results of the last 100 pulses as well as of the pulse parameters are stored in Gene Pulser Xcell
memory and are accessible from the Data Management program (Section 3.8).
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