Bio-Rad Gene Pulser Xcell™ Electroporation Systems User Manual

Page 42

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When the necessary parameter values have been specified, a flashing “P” appears in the character
space in the lower right corner of the LCD display indicating that the pulse button on the Gene
Pulser Xcell is active and that a pulse may be delivered.

To use the program:

Press Pulse to deliver the pulse at the specified voltage.

After the pulse, the Protocol Results screen is displayed; use the Left and Right Arrow keys to
toggle between the Protocol Results screen and the last Protocol Detail screen.

From the Protocol Detail screen, another pulse at the same voltage may be delivered by
pressing Pulse. Press Enter to bring the cursor on the screen; the cursor will appear at the
right character space of the first parameter (the voltage). To increase the voltage by the
specified increment value, press the Right Arrow key; to decrease the voltage by the
specified value, press the Left Arrow key. Press Pulse to deliver another pulse.

3.8 Data Management

This section of the program allows the user to view the parameter values and output of the last 100 pulses.
Data for each pulse is presented on a single screen; examples are given below. Each data set includes the
date and time of the pulse, an identifier for how the pulse was programmed, the set parameters, and the
delivered pulse. The pulses are displayed in chronological order or in reverse chronological order and are
logged by date and time. (Check that the date and time are correct under User Preferences (Section 3.10,
Setting the Clock).) Selecting chronological order displays the earliest pulse in the data bank or, if a specific
date was selected, the earliest pulse from the specified date. Selecting reverse chronological order displays
the latest pulse in the data bank or, if a specific date was selected, the last pulse from the specific date.
This is a read-only file. No changes are permitted to this file.

From the Home screen (Figure 3.2) press 8 or use the Up and Down Arrow keys to highlight “Data
Management”, then press Enter to display the Data Management screen (Figure 3.28).

Press 1–4, then Enter to select the data to view. Selecting 3 or 4 brings up a Date screen
(Figure 3.29). Use the keypad to enter a date, then press Enter to select that date and to view the
data. If no data was collected on that date, a screen will appear indicating that no data is present
for that date.

Data Management Screen

Fig. 3.28. Data Management screen.

36

W h a t d a t a d o y o u w a n t t o v i e w ?

A l l d a t a

1 . R e v e r s e c h r o n o l o g i c a l o r d e r

2 . C h r o n o l o g i c a l o r d e r

D a t a f r o m a s p e c i f i c d a t e

3 . C h r o n o l o g i c a l o r d e r

4 . R e v e r s e c h r o n o l o g i c a l o r d e r

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