Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 10)

Coomassie is a trademark of Imperial Chemical Industries. Triton is a trademark
of Union Carbide Corp. Tween is a trademark of ICI Americas, Inc.
*The concentration limits for compatibility with the microassay are 1 /
25
of the
values in Table 1.
8
TBP, 5 mM
TBS (25 mM Tris, 0.15 M NaCl,
pH 7.6), 0.5x
TCEP, 20 mM
Thio-urea, 1 M
Tricine, pH 8, 50 mM
Triethanolamine, pH 7.8, 50 mM
Tris, 1 M
Tris-glycine (25 mM Tris, 192 mM
glycine)
Tris-glycine-SDS, (25 mM Tris,
192 mM glycine, 0.1% SDS),
0.5x
Triton X-100, 0.05%
Tween 20, 0.01%
Urea, 4 M Phenol Red, 0.5 mg/ml
PIPES, 0.2 M
PMSF, 2 mM
Potassium chloride, 2 M
Potassium phosphate, 0.5 M
SB 3–10, 0.1%
SDS, 0.025%
Sodium acetate, pH 4.8, 0.2 M
Sodium azide, 0.5%
Sodium bicarbonate, 0.2 M
Sodium carbonate, 0.1 M
Sodium chloride, 2.5 M
Sodium citrate, pH 4.8 or
6.4, 0.2 M
Sodium hydroxide, 0.1 M
Sodium phosphate, 0.5 M
Sucrose, 10%
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