Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 25)

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If the protein concentration is high
enough, a sample with detergent can
be diluted so that the concentration of
detergent is reduced to 0.1% or less.
Alternatively, the Bio-Rad DC
™ (deter-
gent compatible) protein assay can be
used (catalog #500-0111). The DC
protein assay is a modified Lowry
assay, which works in the
presence of 1% ionic or nonionic
detergent.
3 Is any sample preparation
required?
In general, no. However, the protein
must be solubilized. The sample
cannot be a suspension or an
unfiltered homogenate.
2 My sample contains a
detergent concentration that is
incompatible with the Quick
Start Bradford assay. How can I
assay for protein?
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