Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 24)

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Section 4
FAQs and Troubleshooting
Questions
1 The buffer that I normally use is
not in the list of compatible
reagents. How will I know if it
interferes with the Quick Start
Bradford assay?
Recommendations
It is best to run two standard curves,
one with protein in the same buffer as
your sample and one with protein in
water, and then plot a graph of
protein concentration versus
absorbance. If the buffer does not
interfere, the two standard curves will
have identical slopes. Adding the
buffer or interfering component to the
standards used to construct the
standard curve for the actual protein
assay can compensate for partial
interference.
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