Microplate standard assay, 2 ml microassay cuvette standard dilutions – Bio-Rad Quick Start™ Bradford Protein Assay User Manual
Page 33
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31
Microplate Standard Assay
Standard
Source of
Diluent
Final
Tube #
Volume (µl)
Standard
Volume (µl) [Protein] (µg/ml)
1
20
2 mg/ml stock
0
2,000
2
30
2 mg/ml stock
10
1,500
3
20
2 mg/ml stock
20
1,000
4
20
Tube 2
20
750
5
20
Tube 3
20
500
6
20
Tube 5
20
250
7
20
Tube 6
20
125
8 (blank)
–
–
20
0
2 ml Microassay Cuvette Standard Dilutions
Standard
Source of
Diluent
Final
Tube #
Volume (µl)
Standard
Volume (µl) [Protein] (µg/ml)
1
40
2 mg/ml stock
3,160
25
2
65
2 mg/ml stock
6,435
20
3
30
2 mg/ml stock
3,970
15
4
3,250
Tube 2
3,250
10
5
3,250
Tube 4
3,250
5
6
3,250
Tube 5
3,250
2.5
7
3,000
Tube 6
3,000
1.25
8 (blank)
–
–
3,200
0
4110065A.qxp 9/25/2007 2:39 PM Page 37
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