Bio-Rad Quick Start™ Bradford Protein Assay User Manual
Page 13
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Section 2
Instructions
2.1 Standard Protocol
1.
The standard protocol can be performed in
three different formats, 5 ml and a 1 ml
cuvette assay, and a 250 µl microplate assay.
The linear range of these assays for BSA is
125–1,000 µg/ml, whereas with gamma-
globulin the linear range is 125–1,500 µg/ml.
2.
Remove the 1x dye reagent from 4°C storage
and let it warm to ambient temperature. Invert
the 1x dye reagent a few times before use.
3.
If 2 mg/ml BSA or 2 mg/ml gamma-globulin
standard is used, refer to the tables in the
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