Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 16)

6. Set the spectrophotometer to 595 nm. Zero
the instrument with the blank sample (not
required for microplate readers). Measure the
absorbance of the standards and unknown
samples. Refer to Section 3 for data analysis.
Note: If the spectrophotometer has a
reference and sample holder, the instrument
can be zeroed with two blank samples. If the
effect of buffer on absorbance is required,
zero the instrument with a cuvette filled with
water and dye reagent in the reference
holder.
2.2 Microassay Protocol
1. The microassay protocol can be performed in
two different formats, a 2 ml cuvette assay
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