Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 26)

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4 Does the binding of the blue
dye to cuvettes skew results?
Bio-Rad's disposable polystyrene
cuvettes (catalog #223-9950) are
recommended for the protein assay.
When using quartz cuvettes, the
amount of dye that binds to them is
negligible (Bradford 1976). Therefore,
removal of the residual blue color
between each sample reading is
unnecessary. However, since the
cuvettes may be used for subsequent
procedures, there are several recom-
mended treatments for dye removal:
• Rinse the cuvette with methanol,
or
• Rinse the cuvette with glassware
detergent, followed by ddi water,
or
• Soak the cuvette in 0.1 N HCl for
a few hours, then wash with ddi
water.
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