Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 34)

32
2 ml Microassay Cuvette Dilutions for BSA or
Gamma-Gobulin Standard Sets
Standard Source of Diluent Final
Tube # Volume (µl) Standard Volume (µl) [Protein] (µg/ml)
1 40 2 mg/ml 3,160 25
2 35 2 mg/ml 3,465 20
3 35 1.5 mg/ml 3,465 15
4 35 1 mg/ml 3,465 10
5 35 0.5 mg/ml 3,465 5
6 35 0.25 mg/ml 3,465 2.5
7 35 0.125 mg/ml 3,465 1.25
8 (blank) – – 3,200 0
Microplate Microassay Dilutions for
2 mg/ml BSA or Gamma-Globulin
Standard Source of Diluent Final
Tube # Volume (µl) Standard Volume (µl) [Protein] (µg/ml)
1 10 2 mg/ml stock 790 25
2 10 2 mg/ml stock 990 20
3 6 2 mg/ml stock 794 15
4 500 Tube 2 500 10
5 500 Tube 4 500 5
6 500 Tube 5 500 2.5
7 500 Tube 6 500 1.25
8 (blank) – – 500 0
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